Fig. 6. Effects of misexpression of BR-Z1 on the morphology of the resultant pharate adults. All animals were scored about 120 hours APF, about 24 hours after the expected time of eclosion. (A) Pharate adults of the Z1/Z1; Z1/Z1 line (w1118; 527-5; 708-1) that have two copies of the TNT-Q1-Q2 variant and two of the Q1-Q2 formed after expression at various times during adult development. Note the truncated bristles in the head and thorax after misexpression at 32.5 hours, but in the abdomen only later at 35 hours. Note the transparent pupal-like cuticle in the dorsum with little bristle pigmentation except in parts of the abdomen after misexpression at 48 and 52 hours. (B) Whole mounts of abdominal cuticle formed after expression of the same transgene as in A at the designated time APF. Note the truncated bristles after misexpression at 35 and 39 hours and the formation of bristles with little or no pigmentation after misexpression at 45 and 48 hours. (C) Pharate adult after misexpression of Z1/Z1 (line w1118; 708-1) at 36 and again at 48 hours APF. Note the thin transparent pupal-like cuticle over the entire animal. Some bristles are present on the head and dorsal thorax but are not pigmented. The inset shows a pupal-like wing found in some of these animals.
