Fig. 4. Endogenous RA localization in conditionally rescued Raldh2–/– embryos carrying RARE-lacZ. Staining for expression of RARE-lacZ was performed on Raldh2–/– and wild-type embryos treated with RA every 12 hours from E6.75-E8.25, then examined at E9.5 (A,B) or E10.5 (C,D). f, forebrain; f/m, forebrain/midbrain junction; h, hindbrain; he, heart; m, midbrain; mx, maxillary process; n, nasolacrimal groove; ol, olfactory pit; op, optic vesicle; sc, spinal cord; u, urinary tract; *, truncated forelimb bud.
