Fig. 1. The proliferation and accumulation of CMZ cells is stimulated by insulin, but not by FGF2. (A-D) Vertical sections of the temporal retinal margin that have been labeled for BrdU immunoreactivity. Sections were obtained from eyes that were treated with (A) saline, (B) insulin alone, (C) FGF2 alone, and (D) insulin and FGF2. (E) Histogram demonstrating that the number of BrdU-labeled cells added to the nasal and temporal peripheral edge of the retina is stimulated by insulin, but is not further stimulated by co-application of FGF2. (F-T) Vertical sections of the retinal margin were double labeled with antibodies to Pax6 (green) and Chx10 (red). Tissues were obtained from eyes that received two consecutive daily injections of (F-H) saline or (I-T) insulin alone. Tissues were obtained (F-N) 24 hours or (O-T) 10 days after the final injection. Small arrows indicate cells that are double-labeled for Pax6 and Chx10, arrowheads indicate cells labeled for Pax6 or Chx10 alone, and the large arrows indicate the retinal margin, with the pars plana to the right and more central regions of the retina the left. The boxed areas in K and Q are enlarged in L-N and R-T, respectively. INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars: in D, 50 µm in A-D; in Q, 50 µm in F-K and O-Q.
