Fig. 2. Markers of ganglion cells are expressed in the CMZ after treatment with insulin alone and insulin with FGF2. (A-E) Vertical sections of (A) central retina and (B-E) the retinal margin that have been labeled for neurofilament immunoreactivity. Sections were obtained from eyes that were treated with three consecutive daily injections of (A,B) saline, (C) insulin alone or (D,E) insulin and FGF2. Retinas were processed for immunocytochemistry (A-D) 24 hours after the final injection or (E) 4 days after the final injection. Arrows indicate the CMZ, with the ciliary body to the right and central regions of the retina to the left. Scale bar: 50 µm in A-E. ONL, outer nuclear layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer; NFL, nerve fiber layer. (F) Histogram demonstrating that the number of neurofilament-positive cells in the CMZ is increased by injections of insulin alone and insulin and FGF2, but not by FGF2 alone.
