Fig. 5. Insulin and FGF2 induce the production of Brn3.0-expressing ganglion cells at the retinal margin. Vertical sections of (A-C) central retina and (D-I) the retinal margin were double-labeled for (A,C,D,F; green) neurofilament and (B,C,E,F; red) Brn3.0 or (G,I; green) BrdU and (H,I; red) Brn3.0. Tissues were obtained from eyes that received three consecutive daily injections of (D-F) insulin alone or (A-C,G-I) insulin and FGF2. The large arrow indicates the retinal margin, with the ciliary body to the right and central regions of the retina to the left. The small arrows in A-C indicate a displaced ganglion cell that expresses neurofilament but not Brn3.0, and the small arrows in G-I indicate double labeled cells. INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars: in F, 50 µm in D-F: in I, 50 µm in A-C,G-I.
