Fig. 3. Effect of ß1 integrin deletion on keratinocyte migration in vitro. (A) ß1 fl/fl keratinocytes were infected with an EGFPCre-expressing retrovirus and examined 5 days later. Flow cytometry demonstrates that the ß1 integrin subunit is missing in 50% of EGFP-positive keratinocytes. (B) The GFP positive keratinocytes fail to spread. (C) ß1 fl/fl keratinocytes transduced with chick ß1 integrin. Red, second antibody control; blue, chick ß1 integrin-positive cells. (D) Chick transduced keratinocytes remain spread after infection with the EGFPCre retrovirus. (E) Phase and photograph of ß1 fl/fl keratinocytes infected with EGFPCre at t=0 hours of the movie. In colour are the tracks of two EGFPCre infected (dark and light blue tracks) and uninfected (red and green tracks) keratinocytes. For detailed migration movies, see http://dev.biologists.org/supplemental/.
