Fig. 6. Cell-cell and cell-matrix interactions during wound repair. Immunostaining on sections from the middle of 10-day wounds, with an anti-ß1 integrin antibody reveals expression in basal keratinocytes of the healed epidermis of control mice and confirms the absence of ß1 in the epidermis of K5ß1-null mice (arrows in A,B). Lack of ß1 integrins also results in a disturbance in hemidesmosome localisation, as detected by immunostaining on sections from 5-day wounds for ß4 integrin (red; arrows in C,D). The hyperproliferative epithelium is stained with an anti-keratin 6 antibody (green). G, granulation tissue; HE, hyperproliferative epithelium. This difference in hemidesmosome staining is recapitulated in the staining for the laminin 5 substrate (magenta) to which they bind (arrows in E,F). By contrast, expression of E-cadherin (magenta) is similar in the hyperproliferative epithelium of 5-day wounds of control and K5ß1-null mice (G,H). G, granulation tissue; HE, hyperproliferative epithelium. Scale bars: 50 µm.
