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Fig. 6. Brn3a expression is normalized towards wild-type levels in Brn3a heterozygotes. Brn3a mRNA levels were assayed in E13.5 embryonic tissues by RT-PCR; the mean ±s.d. of triplicate assays are shown. (A) Brn3a expression in the intact E13.5 head, including Brn3a neurons in the midbrain, hindbrain and cranial sensory ganglia. (B) Brn3a expression in isolated trigeminal ganglia. (C) The location of tissue samples dissected from the E13.5 midbrain tectum, in which Brn3a expressing neurons are labeled by immunohistochemistry (green). aq, aqueduct; pml, post-mitotic layer; ne, neuroepithelium; tec, tectum; teg, tegmentum. (D) Brn3a expression in the isolated E13.5 tectum.





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