Fig. 1. Glial cells fragment in the microchaete lineage. (A,B) A live neu^P72 UAS-H2B::YFP pupa was observed by time-lapse confocal microscopy. Time (hours:minutes) after puparium formation (APF) is shown at the bottom right of each panel. (A) A pI cell was followed from its initial division (at about 17:15 hours APF) until pIIIb division (at about 22:00 hours APF). Two hours later, the glial cell began to fragment (arrow at 23:53 hours APF). (B) Glial cell fragmentation in another bristle lineage from the same pupae as in A. Note that, in this case, the glial cell began to fragment before the division of pIIIb (at 21:33 hours APF and 22:23 hours APF, respectively). (C) Image taken from an in vivo observation of a scabrous-GAL4 UAS-nlsGFP pupae at 23:45 hours APF. The glial cell was identified by the size of its nucleus and lineage criteria. Note glial cell fragments (arrowhead). With this GFP construction, fragmentation was discernible only in rare situations. (D) Glial cells fragment in wild-type pupae. Sensory organ cells were stained with Senseless (green) and Repo (blue) antibodies to identify sensory and glial cells, respectively. Note that the TUNEL-positive cell (red) nearby a sensory cluster is also Senseless-immunoreactive (arrow in D). Also note that Repo-positive glial cells did not co-exist with other TUNEL-positive cells in the same cluster (arrowhead in D). In A and B, each image corresponds to the merging of 12 horizontal confocal optical sections. pI, primary precursor cell; pIIa and pIIb, secondary precursor cells a and b; pIIIb, tertiary precursor cell b; g, glial cell, n, neurone; so, socket cell; sf, shaft cell; st, sheath cell. Anterior is upwards and the view is horizontal.

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