Fig. 3. Inhibition of apoptosis did not prevent the loss of the glial cell. Time-lapse confocal analysis of a neu^P72 UAS-H2B::YFP UAS-p35 living pupae. Hours:minutes APF is shown at the bottom left of each panel. The glial cell (arrowheads) was maintained alive after overexpression of p35. (A-C) Horizontal views. (A'-C') Lateral views of the corresponding horizontal views. The glial cell remained near the cluster for about 4 hours after it was produced (A,A'). After this period, the glial cell moved away from the cluster and fell down towards the internal cavity (lateral view at 24:15 hours APF, B'). At about 25 hours APF, the glial cell could not be observed anymore. Note that fragmentation was never observed. Each image corresponds to the merge of 18 horizontal confocal optical sections. Lateral projections were reconstructed from the horizontal sections at the positions showed inside the brackets. Broken line in A'-C' denotes the dorsal surface. Anterior is upwards in A-C.

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