Fig. 6. The apoptosis of the glial cell is independent of glial identity and of pros expression. (A,B) gcm^e1/gcm^e1 somatic clones at 24 hours APF are shown. Clones were detected because of their lack of GFP staining (green), their limits are shown with a white line (in A). Sensory organs cells were detected with anti-Cut antibodies (also in green). Neurones were identified by Elav-immunoreactivity (red). (A) At 24 hours APF, two types of sensory clusters were observed inside the gcm^e1/gcm^e1 clone: four-cell clusters (arrow) and five-cell clusters (arrowhead). In these cases, an extra Elav-positive cell could be detected (arrowhead). Note that clusters with two Elav-positive cells were never observed outside the clone. (B) Detailed view from another gcm^e1/gcm^e1 clone. The arrowhead shows Elav-positive cell fragments next to a four-cell cluster. (C,D) pros¹⁷/pros¹⁷ somatic clones at 24 hours APF are shown. Clones were detected because of their lack of GFP staining (green), their limits are shown with a white line. Sensory organs cells were detected with anti-Senseless antibodies (in green, C,D) and with anti-Cut antibodies (also in green, D). (C) At 24 hours APF, some cell fragments co-labelled with Senseless and Repo (red) were detected within pros¹⁷ clones (arrow), other fragments did not show any Repo staining (arrowhead). (D) Glial cell fragmentation could be detected using TUNEL staining (red) inside pros¹⁷ clones as well as outside the clones (arrows). Anterior is upwards and the view is horizontal.

Return to article