Fig. 4. TLE1 synergizes with SIX3 and SIX6 in over-expression assays. Dorsal views of stage 24 embryos injected with either SIX3 (A,B,E,F) or SIX6 (C,D) mRNA alone (A,C,E) or in combination with TLE1 (B,D,F). Concentrations are indicated in the panels. Embryos were hybridised to detect Rx2 expression. Note that 10 ng/µl of SIX3 or SIX6 are not effective in inducing ectopic Rx2 expression (A,C). Co-injection with TLE1, clearly boosts SIX3 and SIX6 activity and induces ectopic Rx2-positive tissue in the midbrain (arrows in B and D). The phenotype induced by higher doses of SIX3 (E, ectopic midbrain expression. arrow) was also enhanced by TLE1 co-injections (F), leading to the striking appearance of additional separate patches of ectopic Rx2 expression (arrowheads in F). Scale bar: 0.1 mm.

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