Fig. 2. Ectopic wg-vg co-expression driven by vg-G4 causes the transformation of eye territories into wing blade with cuticular structures (A) and gene expression (C,D) characteristic of wing blade. The expression of vg-G4 is detected in the eye imaginal disc by the simultaneous mobilisation of UAS-GFP (GFP) (B-D). Scale bars: 0,1 mm. (A) Observe chaetae (black arrowhead) and wing blade trichomes which appear when we ectopically express vg in the eye. (B) In the wild-type eye imaginal disc, vg-G4 (GFP) and wg (red) are expressed in different territories. High levels of wg expression are detected in the poles of the eye, whereas vg-G4 is expressed in the equatorial line in the eye. (C) Notice the autonomous ectopic expression of nub (red; white arrowhead) in transformed eye cells caused by wg-vg co-expression (GFP). (D) Ectopic expression of vg (red; white arrowhead) in eye transformations caused by the wg-vg co-expression. Notice that the level of wg expression is very low in the transformed territories (white arrowhead).

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