Fig. 3. Adult phenotypes (A) and imaginal disc gene expression (B-F) caused by the overexpression of vg in clones initiated in the wing blade and wing hinge. Adult clones are labelled with f and delimited by a broken red line. In the imaginal discs, vg clones are associated with green fluorescent protein (GFP). Scale bars: 0.1 mm. (A) Tubular outgrowth including a clone of vg (red arrowhead). The age of clone initiation is 36±12 hours AEL. In the inset a detail of the same clone is shown. (B) In the wing hinge, vg overexpression in clones (green) displaces the rings of wg expression (red) by several cellular diameters. The age of clone initiation is 60±12 hours AEL. (C) In the wing hinge, the vg overexpression in clones (green) non-autonomously activates the expression of Dll (red) and autonomously represses Hth expression (blue). The age of clone initiation is 60±12 hours AEL. (D) In the wing hinge, vg overexpression in clones (green) (white arrowhead) autonomously activates the vg QE-lacZ reporter (red). The age of clone initiation is 60±12 hours AEL. (E) vg overexpression in clones (green; white arrowhead) never activates the vg BE-lacZ reporter (red) reporter in the wing imaginal disc (or in other tissues). The age of clone initiation is 60±12 hours AEL. (F) In all studied tissues, vg overexpression in clones (green; white arrowhead) autonomously activates the sd-lacZ reporter (red). The age of clone initiation is 60±12 hours AEL.

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