Fig. 4. Adult `mixed' tissue phenotypes (A,B) and imaginal disc expression (C,D) caused by the ectopic clonal expression of vg in the notum. Adult clones are labelled with f and delimited by a broken red line. In the imaginal discs, vg clones are associated with GFP. The age of clone initiation is 36±12 hours AEL. Scale bars: 0.1 mm. (A) Clone of vg ectopic expression in the notum initiated at 36±12 hours AEL; high magnification is shown in B. The clone territory contains trichomes of wing blade (red arrowhead) and notum chaetae in a `salt and pepper' distribution. Notice the differences between trichomes with wing blade characteristics (red arrowhead) contained in the clone with notum trichomes (black arrowhead). (C) Clones of vg ectopic expression (green) in the notum that simultaneously contain cells expressing [dorsal territories (d)] and non-expressing [ventral territories (v)] the ap-lacZ reporter. Notice that endogenous wg expression in the notum appears displaced (yellow arrowhead), whereas wg expression is enhanced within the clone (white arrowhead) at the confrontation between cells expressing and non-expressing ap-lacZ, as occurs in the wild-type wing margin. (D) Adult mixed tissues are correlated, in imaginal discs, with the autonomous expression of wing blade genes such as bs (red). Notice that the expression of bs is non-autonomously induced outside the clone (green; white arrowhead), as well as partially induced within the clone.

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