Fig. 2. Structure of injected transgenes, and DPY-22 and TRAP230 proteins. (A) Graphical depiction of the PCR fragment amplified from cosmid F47A4 used for rescue experiments. PCR was used to amplify the region between 21115 and 6044 of F47A4. This region contains the entire predicted dpy-22 open reading frame (18942-7241) and parts of the last two exons from F47A4.3. (B) Structural organization of DPY-22 and TRAP230 proteins. Numbers above the proteins refer to amino acid positions. Hatched area represents a glutamine-rich region. Gray areas represent regions of amino acid identity between DPY-22 and TRAP230, as defined by BLAST, with the percentage identity listed below. The positions of the dpy-22 nonsense mutations used in this study are indicated. (C) Structure of DPY-22::GFP fusion proteins encoded by transgenic constructs. DPY-22::GFP is a fusion of the full-length wild-type DPY-22 to non nuclear-localized GFP. DPY-22 2548::GFP and DPY-22 2141::GFP are fusions between the first 2548 and 2141 amino acids of DPY-22, respectively, to non nuclear-localized GFP. Black boxes indicate putative nuclear localization sequences predicted by PROSITE. Hatched area represents a glutamine-rich region. Gray areas indicate GFP amino acids.

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