Fig. 4. p27^Xic1 can enlarge the myotome independently of its ability to arrest the cell cycle. One cell of two-cell stage embryos were injected with (A,E) 45 pg p27^Xic1, (B,F) 15 pg p27^Xic1 NT, (C,G) 50 pg p27^Xic1 CT or (D,H) 50 pg p27^Xic1 35-96 and ß-gal as a tracer (light blue, injected side to left) and allowed to develop until stage 15 (A-D) or 22 (E-H). Dorsal views with anterior downwards (midline indicated by broken white line) demonstrate that phosphohistone-H3 staining (purple) is reduced after injection of all four p27^Xic1 constructs (A-D). Transverse sections stained with an antibody against muscle actin (dark blue) indicate that full-length p27^Xic1 (A) and p27^Xic1 NT (B) can increase the size of the myotome (arrows), while p27^Xic1 CT (C) and p27^Xic1 35-96 (D) have no effect.

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