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Fig. 7. Ablation of p27Xic1 causes excess proliferation and loss of differentiated muscle. Embryos were injected in one cell of two-cell stage embryos with (A,A') 10 ng Con Mo or (B-C') 10 ng p27Xic1 Mo and ß-gal (light blue in A',B') (injected side upwards) and allowed to develop until stage 21 (A-B') or stage 26 (C,C'). Embryos were stained for phospho-histone-H3 (purple in A',B'), longitudinally sectioned and stained with an antibody against muscle actin (MA) (red in A,B,C'). DNA is stained with Hoechst (blue, A,B,C).





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