Fig. 2. Embryos depleted of p27^Xic1 protein fail to produce differentiated primary neurones. (A) Western blot for endogenous p27^Xic1 protein levels on stage 18 embryos that were injected with 20 ng Con Mo or p27^Xic1 Mo. Cytoskeletal-ß-tubulin demonstrates equal loading. Con Mo injection has no effect on any of the neural markers examined (B, data not shown). (C-F) p27^Xic1 Mo-injected embryos were analysed for expression of X-NGNR-1, XMyT-1, NeuroD and Nßtub (purple) by whole-mount in situ hybridisation, injected side towards the left (ß-gal, light blue). Injection of 20 ng p27^Xic1 Mo has no effect on X-NGNR-1 (C) but ablates XMyT-1 (D), NeuroD (E) and Nßtub (F) expression.

Return to article