Fig. 5. Nkx3.1 expression is not reduced in Hoxb13 homozygous mutants. (A) Western blots using an anti-Nkx3.1 antibody and an anti-ventral prostate protein antibody were performed on samples of protein extracts from ventral duct cells where secreted proteins were first removed. Nkx3.1 levels are not reduced in mutant ventral prostates, while antibodies to the ventral prostate proteins show complete absence of ventral prostate-specific proteins in the mutant VP. (B) Western blots using an anti-Nkx3.1 antibody and an anti-ventral prostate protein antibody on total protein samples show that Seminal vesicles but not anterior prostates secrete p12. Furthermore, expression of p12 in the seminal vesicle is not affected in Hoxb13 homozygous mutants. (C) Coomassie Blue stained gel of total proteins shows that homozygous mutant ventral prostates share many common bands with control anterior prostates (black arrowheads), but also display some prominent ventral-specific bands (asterisks). SV, seminal vesicle; AP, anterior prostate; VP, ventral prostate, B, bladder.

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