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Fig. 9. FRL-1 expression in neuroectoderm at the late gastrula stage.
(A,B) Whole-mount in situ hybridization revealed the spatial expression
patterns of FRL-1. (A) Lateral view of a stage 10 embryo; right side
is dorsal. FRL-1 was ubiquitously expressed in the early gastrula.
(B) Lateral view of a stage 12 embryo; right side is dorsal. Transcripts of
FRL-1 are concentrated in the neuroectoderm. (C) RT-PCR analysis of
chordin-injected ectoderm. Misexpression of chordin induced
the expression of FRL-1 in the absence of the mesodermal marker,
Xbra.
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