Fig. S1. Mutations in the spr-3 gene de-repress the expression of the hop-1 gene in the L1 stage. (A) hop-1 mRNA concentration, with ama-1 control, at the L1 stage from N2, sel-12(ar171) and most sel-12(ar171) spr-3 strains. (B) The relative expression of hop-1 for the bands shown in A, after adjusting for equal loading.

Fig. S2. Mutations in spr-3 and spr-5 derepress the expression of the hop-1 gene in the L1 stage in a sel-12(+) background. hop-1 mRNA with ama-1 loading control in the L1 stage for N2 (wild type), spr-5(by101), spr-3(by136) and spr-5(by135). All spr strains are in a sel-12(+) background. Below the bands is listed the -fold expression of hop-1 compared with N2, after correction for equal loading. Experimental methods are the same as for the Fig. 7 in the paper.

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