Fig. 5. The cloning of spr-4. (A) The genetic position of spr-4 (see Table S1 at http://dev.biologists.org/supplemental/). (B) Physical map near C09H6.1. spr-4 was mapped close to but to the right of byP7 (SNP v120a11.s1{at}186). The extent of the 12 kb PvuII rescuing fragment is shown. (C) The predicted SPR-4 protein aligned with the 5' end of C28G1.4 and the 3' end of G28G4.4. Identical amino acids are highlighted in black and similar amino acids are highlighted in gray. The 18 putative C2H2 zinc fingers of SPR-4 are underlined, and regions that could act as nuclear localization signals are boxed. The two amino acids present only in the alternatively spliced form of SPR-4 are overlined and labeled at. Mutations are indicated with an asterisk. (D) Alignment of SPR-4 with REST (Homo sapiens). Identical amino acids are highlighted in black and similar amino acids are highlighted in gray.

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