Fig. 7. Inhibition of Xgly4 translation disturbs the membrane accumulation of the Xdsh protein in the dorsal mesoderm. (A-D) The Xdsh protein accumulated at the cell membrane of dorsal mesoderm cells during gastrulation (data not shown). Injection of the dominant-negative Xwnt11 mRNA (dn-Xwnt11; 500 pg; B), Xgly4Mo (41 ng; C), or Xgly4 mRNA (500 pg; D) disturbed the Xdsh protein localization. The accumulation of Xdsh protein was not affected by the control morpholino oligonucleotide (A). The smeared staining in cytoplasm was observed only in co-injected cells but not in cells injected with Xdsh alone, which had previously been injected with Xgly4Mo only in one side of dorsal blastmeres at the four-cell-stage (data not shown). (E) The intensity of Xdsh staining in the cytoplasm and membrane in each cell was quantitated by using NIH image.

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