Fig. 2. The feronia mutant is semisterile and shows reduced transmission through the female gametophyte. (A) Wild-type siliques show full seed set. (B) In feronia siliques only about half of the ovules develop into seeds, the others remain unfertilized (arrows). (C) Southern blot using Ds 5' end-specific probe indicates a single Ds insertion in the feronia mutant. The observed fragment sizes are consistent with the expected sizes after restriction with EcoRI (3.2 kb) and HindIII (2.0 kb). (D) Transmission efficiency (TE) of the kanamycin resistance gene, which is tightly linked to feronia, through wild-type and feronia gametophytes [TE=(kanamycin resistant seedlings)/(kanamycin sensitive seedlings); progeny of reciprocal out-crosses to the wild type]. In feronia the transmission through the male gametophyte is reduced to 78.5% and through the female gametophyte to 14.5%.

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