Fig. 4. Supernumerary PTs enter mutant feronia FGs. (A,D-F) Cleared whole-mount preparations. (B) Ovule stained with Aniline Blue to visualize the PT. (C) Confocal micrograph with transections in z-axis. A and B show the same ovule in the same focal planes. At least two PTs (arrows, PT1 false coloured in red, PT2 in green) enter the ovule at the micropyle (arrowhead). (C) Two pollen tubes (pt1, pt2) enter the ovule and grow through the micropyle towards the embryo sac. The top and the side panel show the transections of the confocal image stack in z-axis along the lines shown in the main panel. Arrows in the transections mark the two PTs reaching the FG. (D,E) Successive arrival of PTs at ovules at 12 HAP. (D) The first PT (arrow, false coloured in green) has already entered the micropyle. (E) Different focal plane of the same ovule. The second PT approaches the micropyle (arrowhead, false coloured in red). The arrow indicates the first PT entering the ovule. (F) Two PTs (arrows) have invaded (arrowhead) and entered the FG and proceeded into the central cell (cc). This specimen was derived from a tetraploid feronia plant. Scale bars: 30 µm.

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