Fig. 10. (A-F) Expression pattern of GmNotch. Flat preparations of whole embryos (A,B) and leg segments (C-F) stained for a DIG-labelled GmNotch probe. (A,B) At stage 4 GmNotch is expressed in all neuroectodermal cells (arrows) at heterogeneous levels. (C) At stage 1 GmNotch is expressed in segmentally repeated stripes, but shows a stronger expression in the ventral neuroectoderm (arrow). (D) During the first wave of neural precursor formation, GmNotch is expressed uniformly in the head segments and the first three leg segments (arrow). (E) The uniform expression resolves into a heterogeneous expression pattern before formation of the next wave of invagination sites. The arrowhead points to a region of low GmNotch expression, a higher expression is visible in the adjacent region (arrow). (F) GmNotch expression has extended posteriorly and still shows a heterogeneous expression pattern in the neuroectoderm. The expression in the limb buds probably corresponds to the formation of mesodermal tissue (arrowhead). ant, antennal segment; gz, growth zone; l1 to l5, leg segments 11 to 15; md, mandibular segment; mx, maxillar segment; pmd, premandibular segment; pmx, premaxillar segment. Scale bars: 120 µm in A,B; 50 µm in C-F.

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