Fig. 3. (A-J) Sequential formation of invagination sites in the myriapod. Confocal micrographs of flat preparations of whole embryos (A-E) and the first two leg segments (F-J). Anterior is towards the top, the midline towards the left in F-J. (A,F) No invagination sites are visible at stage 1. (B,G) When the limb buds form at stage 2, the first invagination sites arise in the medial region of each hemisegment (arrowhead in G). (C,H) New invagination sites arise anterior, posterior and in-between the existing invagination sites (arrowheads in H) during the second wave of neural precursor formation. (D,I) At early stage 4, the next wave generates invagination sites that form a semicircle around the central region where invagination sites have already formed (arrowheads in I). (E,J) At stage 5, the embryo curves inwards and the ventral neuroectoderm stretches along the mediolateral axis. ant, antennal segment; l1 to l7, leg segments 1 to 7; md, mandibular segment; mx, maxillar segment; pmd, premandibular segment; pmx, premaxillar segment. Scale bars: 120 µm in A-E; 50 µm in F-J.

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