Fig. 4. (A-F) Proliferating cells are associated with invagination sites. Confocal micrographs of flat preparations of embryos stained with phalloidin-rhodamine (red) and anti-phospho-histone 3 antibodies (green). Anterior is towards the top and the midline towards the left in D-F. (A-C) Single mitotic cells are associated with invagination sites that have already formed. The arrow in A points to an invagination site. The arrow in B points to a mitotic cell located at the same position as the invagination site indicated in A. The overlay of A and B is shown in C. (D-F) During formation of the first invagination sites in the central region of the hemisegment (arrowheads and arrow in D), mitotic cells are located at the centre of the invagination sites (arrowheads in E and F) or close to the invagination site (arrows in E and F). l1 to l2, leg segments 1 to 2. Scale bars: 50 µm in A-C; 10 µm in D-F.

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