Fig. 5. (A,B) Comparison of the deduced amino acid sequences of the conserved domains of GmASH and GmDelta, and their relationships to the same protein regions of other species. (A) The alignment compares the basic domain, the two helices and the loop region of GmASH with the bHLH region of another diplopod, two insects, two vertebrates and two spider sequences. Note that the loop region of Glomeris ASH is reduced to the same extent as in the spider and vertebrate bHLH domains. The GmASH bHLH domain shows the highest similarity to the same region of the millipede Archispirostreptus sp. See text for further details. (B) The alignment compares the highly conserved DSL domain (Delta, Serrate, Lag2) of GmDelta with the DSL domains of the same species used for the alignment in A. The GmDelta DSL domain shows the highest similarity to the same protein region of Anopheles gambiae (67% identical amino acids), Archispirostreptus sp. and Cupiennius salei Delta1 (65% identical amino acids each). Ag, Anopheles gambiae; As, Archispirostreptus sp.; Cs, Cupiennius salei; Gm, Glomeris marginata; Hs, Homo sapiens; Mm, Mus musculus.

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