Fig. 4. Apoptotic cells are easily detected in the DRG, but not in the gut. (A,B) Activated caspase 3 immunohistochemistry easily identifies apoptotic cells in E12 DRG (A, white arrows), but not in the E12 ENS (B). Auto-fluorescent nucleated red blood cells are also seen (red arrows) in both the gut and DRG. (C,D) Sections of the gut at E14 (C) and P0 (D) were stained with antibodies to activated caspase 3 (Cy3 secondary antibody) and with antibodies to PGP9.5 (FITC secondary antibody) to show ENS precursors. Activated caspase 3 stained cells were not seen within the ENS at any age examined. Scale bars: in A, 100 µm for A; in B, 100 µm for B-D.

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