Fig. 7. Removal of both Fgf3 and Fgf8 signaling reduces the number, but does not eliminate Dlx3b-expressing otic cells. (A,B) Early expression of dlx3b in cells of the presumptive otic placode (A) is reduced in fgf8^– mutants injected with fgf3-MO (B; n=18). (C-F) Later, at prim-6 stage (25h), dlx3b-expressing cells persist in fgf8^– embryos injected with fgf3-MO (D) and the -Dlx3b antibody shows that the residual Dlx3b-expressing cells are scattered (F), rather than being organized into an otic epithelium (E). Similar results were obtained in 3/15 injected mutants. (A,B) Dorsal view, anterior towards the left; (C-F) side view, anterior towards the left and dorsal towards the top. Scale bar: 100 µm for A-D; 25 µm for E,F.

Return to article