Fig. 4. Deficient cumulus mucification in Tnfip6^–/– females. (A-C) Hematoxylin stained preovulatory follicles in the three Tnfip6 genotypes 48 hours after the PMSG treatment (before hCG injection). (D-F) Hematoxylin stained preovulatory follicles 10 hours after induction of ovulation by hCG injection. (G-L) Localization of hyaluronan (green) in preovulatory follicles 10 hours after hCG injection. Low (G-I) and high (J-L) power magnifications are shown. White arrowheads in L point to cell surface hyaluronan. Nuclei are stained blue by DAPI. Scale bars: 100 µm (A-I) and 50 µm (J-L).

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