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Fig. 7. TNFIP6 is necessary for the transfer of heavy chains (HCs) from I ${alpha}$ I and P ${alpha}$ I to hyaluronan. (A) Tnfip6^–/– mice lack HCs covalently transferred to hyaluronan in their ovaries. Western blot of Streptomyces hyaluronidase-digested ovarian extracts are shown. Open arrowheads indicate dimers or clusters of HCs located close to each other on a single hyaluronan chain and resistant to hyaluronidase digestion. (B) TNFIP6 facilitates the covalent transfer of HCs from I ${alpha}$ I and P ${alpha}$ I to hyaluronan in vitro. Note the formation of a high molecular mass hyaluronan-HC complex (HA-HC) in lane 3 in the presence of recTNFIP6, and the sensitivity of this band to hyaluronidase (h'ase) in lane 4. In both blots, serum with or without chondroitinase ABC (ch'ase) digestion was used as reference for I ${alpha}$ I, P ${alpha}$ I and their HCs.

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