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Fig. 2. Ectopic Wnt1 expression in the cortical hem and cortical ventricular zone of Emx2-null mutant mice. Whole-mount in situ hybridization analysis of Wnt1 expression in 12.5 dpc wild-type embryos showed normal expression of Wnt1 within the roof plate of the neural tube and extending to the rostral diencephalon (A). In addition to the wild-type pattern, Emx2–/– embryos (B) exhibited strong ectopic expression of Wnt1 within the dorsomedial telencephalon, cortical hem organizer region and in the diencephalon (arrows). A dissected coronal view (C) of same embryo at the level of the broken line in B showed ectopic Wnt1 within the cortical hem, hippocampal primordium and cortical ventricular zone (arrow). tel, telencephalon; di, diencephalon; hem, cortical hem; hp, hippocampal primordium; ncx, neocortex.





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