Fig. 6. The effects of spaw inhibition on midline development are restricted to effects on late lefty1 expression. (A-G) Comparison of lefty1 expression in uninjected (u) and Spaw-MO1-injected (10 ng) embryos at various stages. Dig-lefty1 probe. (A,B) Shield stage. (C,D) One to 3 somite stage. (E,F) Seven to 8 somite stage. (G) nineteen to 22 somite stage. (A,B) View onto blastoderm, dorsal is downwards. (C-F) Lateral views, anterior towards the top. (G) Lateral views, anterior towards the upper right. Arrowheads indicate presence (left) or absence (right) of asymmetric lefty1 expression in diencephalon. Asterisks indicate presence (left) or absence (right) of lefty1 in posterior notochord. (H,I) Comparison of ntl expression in uninjected (u; left in H, right in I) and Spaw-MO1-injected (10 ng; right in H, left in I) embryos at shield and 6-7 somite stages. Dig-ntl probe. (H) Arrowheads indicate ntl expression in dorsal shield and forerunner cells. (I) Views of dorsal midline, anterior is towards top.

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