Fig. 1. foi mutants are blocked in gonad coalescence. (A,B) Stage 14 embryos immunostained with an anti-VASA antibody (brown) that labels the germ cells. Dorsal view, anterior left. (A) Wild-type embryo. Note that the germ cells have properly coalesced into the embryonic gonad. (B) foi mutant embryo. The germ cells have aligned on either side of the embryo, but have failed to coalesce into the gonad. (C) Confocal microscopy image showing the gonad region of a stage 14 foi mutant embryo double immunolabeled to reveal the germ cells (anti-VASA, red) and the gonadal mesoderm (anti-EYA, green). Note that although gonad coalescence is blocked, the germ cells have migrated to, and are correctly associating with, the gonadal mesoderm.

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