Fig. 8. Spatially distinct populations of epiphysial neurones show different requirements for flh, ash1a and ngn1. Dorsal (A-D) and lateral (E-G) views of brains with anterior at the top (A-D) or to the right (E-G), showing expression of isl1 at 24 hours in wild-type (WT), flh-mutant and ash1a-morphant embryos, and a flh mutant injected with ash1a MO. In E-G, black arrowheads indicate the limits of the epiphysis, which is marked by a line; white arrowheads indicate the nucleus of the posterior commissure. In the ash1a morphant, remaining neurones are located posteriorly (F). The injection of ash1a MO into the flh mutant leads to the loss of the remaining isl1-positive neurones (D). Scale bar: 10 µm.

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