Fig. 2. Ncad is required for lamination and structural integrity of retinal cells. (A-D) Plastic sections of 3 dpf wild-type (A), pac^rw95 (B), pac^rw95/fr7 (C) and pac^fr7 (D) retinae. The partial fusion of the RGC and amacrine cell layers is indicated in the pac^rw95 retina (B, red asterisks). (E-H) High magnification of the INL in wild type (E), pac^rw95 (F), pac^rw95/fr7 (G) and pac^fr7 (H). A patch of the IPL adjacent to the bipolar cell layer is indicated in the pac^rw95 retina (F, broken red square). (I-L) High magnification of the ONL in wild type (I), pac^rw95 (J), pac^rw95/fr7 (K) and pac^fr7 (L). Some photoreceptors show irregular shape and abnormally aggregate in pac^rw95 (J, red broken parentheses). The photoreceptor layer is wavy and forms rosette structures in pac^rw95/fr7 (K, broken red square). In pac^fr7, cells adjacent to the pigmented epithelium do not display photoreceptor-specific columnar shape (L, red arrowheads). (M-P) BODIPY-ceramide labeled living 48 hpf wild-type (M), pac^rw95 (N), pac^rw95/fr7 (O) and pac^fr7 (P) retinae. In wild type, the IPL forms at the interface between the RGC layer and INL. By contrast, patches of IPL neuropil are observed in the amacrine cell layer of pac^rw95 eye. In the pac^rw95/fr7 retina (O), patches of INL (white arrowheads) are more irregular and the OPL is also wavy (open white arrow). White asterisks show the CMZ. a, amacrine cell layer; b, bipolar cell layer; CMZ, ciliary marginal zone, h, horizontal cell layer; inl, inner nuclear layer; ipl, inner plexiform layer; pe, pigmented epithelium; pl, photoreceptor layer; rgl, retinal ganglion cell layer.

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