Fig. 4. Ncad is required for maintenance of adherens junctions in the retina. (A,B) Twenty-eight hpf wild-type (A) and pac^fr7 (B) retinae labeled with rhodamine-conjugated phalloidin, which stains actin microfilaments (red). In wild type, strongly stained actin foci are visible in the apical termini of retinal cells, closely associated with the pigmented epithelium (A, white arrowheads). In the pac^fr7 eye, actin foci are normally localized in the dorsal retina (white arrowheads), while the line of actin foci (white arrows) is detached from the pigmented epithelium (broken lines) in the ventral retina and localization of actin foci is completely disrupted in the most ventral cells (B, white asterisk). (C,D) Twenty-eight hpf wild-type (C) and pac^fr7 (D) retinae labeled with anti--tubulin antibody, which stains centrosomes. Sections shown in B,D are serial sections. In the wild-type retina, the centrosomes are localized in the apical termini of retinal neuroepithelial cells (white arrowheads). In pac^fr7, the centrosomes fail to be localized apically in the ventral retina (white arrows) and their localization is completely random in the most ventral region (D, white asterisk). In the dorsal region, position of the centrosomes seems to be normal (white arrowheads). (E,F) Plastic sections of 24 hpf wild-type (E) and pac^fr7 (F) retinae labeled with anti-phosphorylated histone H3 antibody, which stains proliferating cells in late G2 and M-phase. In the wild-type retina, dividing cells are localized to the apical surface of the neural retina (white arrows). In pac^fr7, proliferating cells are positioned far from the apical surface in the ventral retina (F, red arrows), but normal in the dorsal retina (F, white arrows). (G,H) Forty-eight hpf wild-type (G) and pac^fr7 (H) retinae labeled with anti-phosphorylated histone H3 antibody. The location of dividing cells is random throughout the pac^fr7 retina. dr, dorsal retina; vr, ventral retina.

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