Fig. 3. Identification of POS-1-interacting proteins. (A) In vitro binding of recombinant proteins. (1) GST and MBP::POS-1, (2) GST::POS-1 and MBP::POS-1, (3) GST::MEX-3 and MBP::POS-1, (4) GST::SPN-4 and MBP::POS-1, (5) GST::LET-92 and MBP::POS-1, (6) GST and MBP::lacZ, (7) GST::POS-1 and MBP::lacZ, (8) GST::MEX-3 and MBP::lacZ, (9) GST::SPN-4 and MBP::lacZ, (10) GST::LET-92 and MBP::lacZ. Positive signals were detected only in lanes 25. (B) The SPN-4 protein. The RNP-type RNA-binding domain (RNP) is shown in black. The cDNA sequence of spn-4 has been deposited with the DDBJ (DNA Data Bank of Japan) under the Accession Number AB052819. (C) Genomic structure of the spn-4 gene. Boxes represent exons. The ORF is shown in gray with the RNP region in black. The trans-splice leader SL1 was detected just one base upstream of the first ATG. The genomic region that was deleted in the tm291 mutant is shown.

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