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Fig. 2. Upregulation of Oct3/4 expression in ES cells induces neural marker gene
expression under serum-free LIF-deficient conditions. (A) ZHTc6 cells were
cultured for 8 days in the presence or absence of Tc. Total RNA was extracted
from each culture and subjected to RT-PCR analysis for the genes indicated on
the right. (B) RNA was extracted from each ZHTc6 cell culture on days 2, 4 and
8, and subjected to RT-PCR analysis for Fgf5 expression.
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