|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Altered gene expression in the posterior pharyngeal region of RA-rescued
Raldh2–/– embryos. (A-D) Whole-mount in situ
analysis of Hoxb1 transcripts in E9 (
22 somites) wild-type (A,C)
and Raldh2–/– (B,D) embryos. Profile views of
the whole embryos (A,B) and enlargement of the posterior foregut region after
medial bisection (C,D, inside views). (E,F) In situ hybridization analysis of
Hoxa1 transcripts in the foregut area of E9 wild-type and
Raldh2–/– embryos, respectively (viewed as in
C,D). (G-J) In situ hybridization analysis of Fgf8 transcripts in
E9.5 wild-type (G,I,) and Raldh2–/– (H,J)
embryos. External profile views (G,H) show Fgf8 ectodermal labeling,
whereas internal views after bisection (I,J) reveal the endodermal labeling.
Insets in (I,J) are coronal views which make it possible to distinguish the
ectodermal and endormal labeling. (K,L) In situ hybridization analysis of
Pax9 expression in the foregut region of E9.5 (main panels) and E10.5
(insets: detail of the pharyngeal region caudally to the 2nd arch) wild-type
and Raldh2–/– embryos, respectively. b1-b3,
branchial arches; ec, ectoderm; en, endoderm; fg, foregut; me, mesoderm;
p1-p3, pharyngeal pouches; r4, rhombomere 4; tb, tail bud.
|
