Fig. 10. Yeast two-hybrid interaction test of LIN-11 and LDB-1. Yeast cells were transformed with plasmids pGBKT7 and pGADT7 (1), pGBKT7-lin-11 and pGADT7 (2), pGBKT7 and pGADT7-ldb-1 (3), pVA3 and pTD1 (4), and pGBKT7-lin-11 and pGADT7-ldb-1 (5). In a transformation control (A), all cells can be seen growing on plate that lacks leucine and tryptophan (SD/–Leu/–Trp). However, only positive control (pVA3 and pTD1) and test (pGBKT7-lin-11 and pGADT7-ldb-1) can promote HIS3 expression (B), leading to growth on plate that lacks histidine, leucine and tryptophan (SD/–His/–Leu/–Trp).

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