Fig. 3. Expression pattern of the vulval markers in wild-type and lin-11(n389) vulva. (A,C,E,G,I,K) Nomarski images of the L4 stage vulval cells. (B,D,F,H,J,L) Corresponding images showing GFP fluorescence. Primary lineage cells have been marked with stars and 2° lineage cells with arrows. Anterior is towards the left. (A,B) Wild-type egl-17::GFP expression in the vulC and vulD cells. (C,D) In n389 background presumptive vulC and vulD do not reveal any egl-17::GFP. Instead, weak egl-17::GFP is expressed in all the 1° lineage cells. (E,F) Wild-type cdh-3::GFP expression is detected in all the 1° lineage cells and in the vulC, vulD of the 2° lineage cells. (G,H) n389; cdh-3::GFP animals have no detectable GFP fluorescence in the 2° lineage cells. In this animal, the presumptive vulF cells are expressing weak GFP, whereas vulE reveal no detectable expression. (I,J) Wild-type zmp-1::GFP expression is seen in the vulA and vulD of the 2° lineage. By contrast, n389; zmp-1::GFP animals (K,L) do not express GFP in any of the vulval cells. The bright fluorescence is seen in some uterine lineage cells. Scale bar: 8 µm.

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