Fig. 3. Various subtypes of MB neurons show different dendritic distribution patterns. (A-F) Mid-coronal confocal sections of adult MB calyces that contain a newly hatched larvae (NHL)-generated, flip-out Nb clone (green). Various subtypes of MB neurons are selectively labeled using GAL4-201Y (A-C) or GAL4-c739 (D-F). Note gaps (arrows in F) present between adjacent GAL4-c739-positive dendritic areas (outlined by broken lines). Mixing of dendrites across clonal boundaries is specifically seen in the upper central subregion (arrowheads in D-F). For a given clone, the primary neurites of neurons are localized on the borders (arrows in C) flanking later-derived MB neurites whereas the primary neurites of last-born /ß neurons lie in the center (arrowhead in C). (G-I) Dendrites of GAL4-c739-positive /ß neurons label the calycal fourfold structure (arrowheads), as revealed on the most anterior coronal confocal section of the adult MB calyx. C,F,I are merged from A and B, D and E, and G and H, respectively. Genotypes: (A-C) Hs-FLP/+;UAS>rCD2, y+>mCD8-GFP/GAL4-201Y; and (D-I) Hs-FLP/+;UAS>rCD2,y+>mCD8-GFP/GAL4-c739. Scale bar: 20 µm.

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