Fig. 5. /ß neurons show birth order-dependent dendritic elaboration patterns. Adult single-cell MARCM clones (green) were generated from 4 hours before white pupa formation to 2 hours after white pupa formation (A,E,I), 2-6 hours after white pupa formation (B,F,J), 6-8 hours after white pupa formation (C,G,K), or later (D,H,L). and /ß lobes were counterstained using mAb 1D4 (red) in A-D. Note the pioneer ß axonal branch (arrow in A) extends along the dorsal surface of the ß lobe and stops short of the ß lobe end. (E-H) Enlarged views of the dendritic areas of the single-cell clones in A-D. Calycal regions of multiple single-cell/two-cell clones are shown in I-L. The entire MB calyx was counterstained using mAb nc82 (red) in I. Note that pioneer /ß neurons project long dendritic processes into the fourfold domain (arrows in E,I), while extending short branches around the calyx base (arrowhead). Genotype: Hs-FLP/+;FRTG13,UAS-mCD8-GFP/FRTG13,tubP-GAL80;GAL4-OK107/+. Scale bars: 20 µm.

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