Fig. 7. Anatomy of CCAP neurons and their role in the clock control of eclosion. (A,B) overlap among the projections and arborizations of CCAP-, timeless-, and PDF-containing neurons. (A) Overlap between projections (arrow) of protocerebral CCAP neurons and tim-containing DN2 neurons of the 3rd instar larval brain. (B) Overlap of the synaptic fields (arrow) of tritocerebral PDF (green) and subesophageal CCAP neurons of the pharate adult brain. Asterisk shows ending of the LN[v] clock cells. DN2, dorsal neuron 2; Tri, tritocerebrum. POT, posterior optic tract (containing projections of the LN[v] clock cells). (C,D) Eclosion profiles for KO (black bars) and control (gray bars) populations under (C) LD (12 hours light:12 hours dark) and (D) DD (continuous darkness). The profile for subjective days 4 and 5 is shown. Height of each bar represents the percentage of flies that eclosed within a 2 hour window normalized to the total number of flies that eclosed during that day (indicated separately for each genotype and day). The open and closed horizontal black rectangles (C) show the light and dark phases of the LD schedule, respectively, while the black and gray rectangles in D show those for the LD regime prior to the shift to DD. Collections were performed at the zeitgeiber (ZT) or circadian (CT) times indicated.

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