Fig. 3. Xlim5 impairs the sorting of vegetal cells from animal cells. Dissociated animal cap cells injected with RLDX were mixed with uninjected (A-A'') or Xlim5-injected (1 ng) (B-B'') vegetal cells and allowed to reaggregate. Aggregates were cleared and viewed by confocal microscopy and scored for sorting. A summary of two experiments is shown in C. Dark grey, positive sorting; white, no sorting. (D,E) Timelapse movies of control animal and vegetal cells in sorting assays. (D,D') Animal cells labelled with TRITC were aggregated with unlabelled vegetal cells and filmed by timelapse video microscopy. Two animal cells (arrows and arrowheads) are shown moving through the aggregate from a time point 30 minutes into filming (D) to a time point 3 minutes later (D'). TRITC-labelled vegetal cells aggregated with unlabelled animal cells and filmed over a similar time course do not show any translocation through the aggregate (E,E').

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