Fig. 1. Transgenic mice overexpressing ALK3^QD. (A) Structure of the transgene. The positions of the Hoxb7 promoter, Q233D mutation, the HA tag, and the human ß-globin sequence are shown. (B) Western blot analysis of proteins isolated from mouse kidney at P10 showing expression of HA-tagged ALK3 in TgALK3^QD mice and a marked increase in ALK3-HA levels in TgALK3^QD/QD mice. (C) ALK3^QD-HA expression in TgALK3^QD mice at P10. Expression of the HA epitope was detected by immunohistochemistry using an anti-HA antibody (brown stain). HA was undetectable in the cortex and medulla of control kidneys. In hemizygous (TgALK3^QD) mice, low levels of HA (arrow) were detected in cortical collecting ducts (T) and in medullary collecting ducts but not in glomeruli (G). In homozygous (TgALK3^QD/QD) mice, much higher levels of HA were detected in a small subset of cortical tubules (T) and in medullary collecting ducts (T).

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